Plasmid

Part:BBa_K197038

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-25)

BBb - BseRI/AcuI entry vector

This part is and entry vector in BBb Format. It is flanked by BamHI, BseRI, BglII, and AcuI sites and is used in the 2AB Layered Assembly scheme developed at UC Berkeley. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2472
    Illegal XhoI site found at 1486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
    Illegal NgoMIV site found at 1339
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
origincoE1
resistanceS